Not sure what to do with the 2 remaining harlequins now. They don't seem comfortable in such small numbers but I suspect they don't have long either... im torn between restocking them as i really like their different body shape and colouring etc. or keeping the number of species in the tank down and increasing numbers of existing fish...
Very sorry to read the news about your harlequins,
@Matt.
As to the course of action to take, I'd probably wait to see how the situation pans out over the next few weeks. My x-ray tetras are now down to 3 in number but, following the initial week or two after the most recent death, seem to be integrating well with the 6 harlequins and not showing any signs of distress. Obviously, I wouldn't advocate keeping such a small number if they were younger but they seem quite content/relaxed in the company of the harlequins, as though they're all part of the same gang, possibly because they've all been in one another's company for 3+ years and thus are used to it. Most fish are dotted around singly, in pairs or small groups at various locations around the tank at various times of the day, and sometimes this can be 2-3 harlequins or a 'pairing' of an x-ray and a harlequin or 2 harlequins plus an x-ray, etc. It's only when lights go off that they tend to congregate in species-specific shoals and even that changes after the first half hour or so. Hope that might provide some reassurance. [It will be interesting to see the dynamics when the microdevario kubotais move into the main tank - I'm inclined to think that this new gang may hang around together, while the old gang remain together, rather than the two rasbora shoals intermingling and leaving the 3 x-rays out 'in the cold'.] If, however, your harlequins show signs of distress (eg hiding, not eating) due to being in such small numbers, then perhaps it is worth increasing their shoal numbers.