API Ammonia Test Kit

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Offline Andy The Minion

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API Ammonia test kit
« on: February 24, 2017, 09:52:04 PM »
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I have just had a bit of a shock and got confused while reading up on Ammonia tests.
I use the API test which is labled as a NH3/NH4+ test and this is Total Ammonia Nitrogen (TAN), not a NH3 (Ammonia) test. The difference between the two is rather important because the NH3 (Ammonia) is the dangerous part of TAN while the NH4 (Ammonium) is considered non toxic.
The limit for NH3 is generally taken as 0.02ppm and the amount in a TAN reading determined by the water pH and temperature. For my tank parameters I would theoretically be able to have a TAN reading of 7.5ppm (seems a Hell of a lot, but that is the maths) before the NH3 level hits 0.02ppm.
I obviously don't run at this level, the test read in a colourimeter normally indicates 0.07ppm so by calculation NH3 is 0.00013ppm (that seems a better number)

Well today I saw the the API test use a Salicylate reagent, I googled this and saw that it tests for NH3 so the result would be 375 times more toxic than the bottle label suggests. Eek!

So here is the confusion, if the API colour chart doesn't start to read until 0.25ppm, this is 12.5 times higher than the maximum safe NH3 level I assume that Google or the API instructions are wrong and this IS a TAN test.
Anybody seen an explanation of this ( @Sue I'm looking in your direction  :wave:)

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Offline barneyadi

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Re: API Ammonia test kit
« Reply #1 on: February 25, 2017, 12:14:34 PM »
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I got sent through the following from another forum, does that help at all?


Offline Andy The Minion

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Re: API Ammonia test kit
« Reply #2 on: February 25, 2017, 01:06:56 PM »
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Thanks @barneyadi The Excel I sent you actually already does this calculation - assuming the API Ammonia test gives a TAN value.

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Offline Sue

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Re: API Ammonia test kit
« Reply #3 on: February 25, 2017, 06:54:00 PM »
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Most home tetsting kits are salicylate ones.

Taken from here
Quote
Q: Do the Nessler and Salicylate methods detect ammonia or ammonium?

A: .........salicylate methods have a reaction with molecular ammonia in a basic solution. If the original sample contained ammonium ions, the basic reagents would convert it to molecular ammonia, and then it will react and contribute to the final test result. Therefore, the term “total ammonia” can be used.

In other words, salicylate tests do measure ammonia and ammonium combined because the reagents turn ammonium into ammonia and then test for ammonia, both the ammonia that was always there plus the ammonia made from ammonium.

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Offline Andy The Minion

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Re: API Ammonia test kit
« Reply #4 on: February 25, 2017, 11:32:20 PM »
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Thanks @Sue, this had to be the answer or all our fish would be long dead but it's good to know the reason. So Google was both right and wrong when it said the salicylate tests for ammonia, it does but only after converting the NH4 so the test reads both NH3+NH4

Fish Community Creator Tanks
Bristlenose Plec (2) - Otocinclus (4) - Bulldog Catfish (1) - Panda Cory (12) - Black Phantom Tetra (6) - Black Widow Tetra (6) - Marbled Hatchetfish (7) - Neon Tetra (13) - Red Phantom Tetra (6) - Red Eye Tetra (6) - Angelfish (2) - Snails (12000) -
Note: The user may not necessarily own these fish, these are tanks that they may be building or researching for stocking purposes


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